Dr Jon Golding

Profile
Research Activity
Publications

Professional biography

I did my PhD research at King's College, London.

Post-doctoral positions at King's College London, Guy's Hospital, MRC National Institute for Medical Research, MRC Clinical Sciences Centre (Imperial).

MRC Collaborative Career Development Fellowship in Stem Cell Research.

Research interests

My lab works mainly on improving the effectiveness of the anti-cancer treatments photodynamic therapy (PDT) and radiotherapy. A full list of my research publications can be found at PubMed or Researchgate

A two-year clinical trial of our new combination PDT, in collaboration with the University of Cambridge, began in October 2013. On the basis of the excellent trial results, this has been adopted as a standard treatment option by Cambridge Veterinary School.

PI. Clinical trial of combination photodynamic therapy for equine sarcoids . European Society for Veterinary Dermatology (2013-2015). €20k

PI. Mechanisms of nanoparticle toxicity in normal and tumour cells. Industrial funding (2015-2018). £68k

Co-I. Targeting radiotherapy with DNA binding metal complexes and their attachment to gold nanoparticles. Royal Society of Chemistry (2015). £5k

Co-I. EU Framework 7 grant PEOPLE-2013-ITN ARGENT- Advanced Radiotherapy, Generated by Exploiting Nanoprocesses and Technologies (2014-2017). £3.65M

Patent: Nanoparticles and their uses in medicine. GB1423049.4 (23/12/2014)

YouTube

Northampton Hospital

Northampton Chronicle

Teaching interests

My main teaching interest is in developing global online life sciences education.

SK320 module chair (Infectious disease and public health)

S390 module chair (STEM capstone project module)

Module author for T366 (Engineering on the Nanoscale)

Impact and engagement

External collaborations

I collaborate with:

Clinicians at Milton Keynes University Hospital and Northampton Hospital.
Veterinary surgeons at the University of Cambridge and Nottingham Trent University
Pharmaceutical companies (Midatech Pharma, Glaxo Smith Kline).
New University of Lisbon (RaBBiT program)
ARGENT ITN

International links

As a member of the Affiliated Research Centre Management Group, I work to ensure the excellent and equivalent provision of PhD student training across the world in our network of OU Affiliated Research Centres (ARCs) and to foster research relationships between to OU and ARCs.

Research Activity

Research groups

Name	Type	Parent Unit
Biomedical Research Network (BRN)	Network	Faculty of Science
Neuroscience Research Group	Group	Faculty of Science

Externally funded projects

Development of H19-targeting antisense oligonucleotides for DIPG therapy

Role	Start date	End date	Funding source
Co-investigator	01/Feb/2020	31/Jan/2021	Abbie's Army
Diffuse intrinsic pontine glioma (DIPG) is the most frequent paediatric brainstem malignancy, and it is invariably associated with a dire prognosis [1]. DIPG is characterized by diffuse infiltrative growth patterns and by an invasive phenotype that underpins its inadequate response to both pharmacological and surgical treatments. DIPG response to radio- or chemo- and targeted therapies is short lived at best. Hence, it is of paramount importance to identify new targets for the development of effective therapies. The genomic landscape of DIPG has been recently described as an heterogenous collection of mutations and copy number variations in protein-coding genes. The most frequent mutation occurs at histone H3 lysine 27 (H3K27M) resulting in widespread epigenetic deregulation. Despite intensive research, no effective protein-targeting therapy has been developed for DIPG. Long noncoding RNAs (lncRNAs) are non-protein coding transcripts longer than 200bp. For several decades, lncRNAs have been considered non-functional transcriptional noise. Recent deep sequencing data have revealed that lncRNAs represent a vast and largely uncharted region of the human transcriptome (more than 50,000 unique loci, 100nMol), broad CNS distribution and rapid cellular uptake. Antisense oligonucleotides (ASOs) are clinically approved molecules that target and silence specific RNA sequences. Our group has shown that antisense ASOs can efficiently target oncogenic lncRNAs. In this project, we will to develop lncRNA-targeting ASOs to stop DIPG proliferation, with the final goal of developing personalised therapies for this incurable disease

Improving the efficiency and targeting of radiosensitisers

Role	Start date	End date	Funding source
Lead	01/Feb/2017	31/Jul/2019	Sir John Mason Academic Trust
New University of Lisbon have a doctoral training program called RaBBiT, whereby the student does research towards their PhD in a host lab (in this case at the OU). The student will be a visitor to the OU for 2 years. The Sir John Mason Academic Trust will meet the cost of the project specific consumables and expenses.

Mechanisms of nanoparticle toxicity

Role	Start date	End date	Funding source
Lead	01/Oct/2015	31/Jul/2019	Midatech Ltd
This is a matched funded PhD student project between LHCS and Midatech. This project will discover why certain nanoparticles are selectively toxic to different cell types.

Publications

Journal articles

Galactose:PEGamine coated gold nanoparticles adhere to filopodia and cause extrinsic apoptosis (2019-02-01)
Tzelepi, Konstantina; Espinosa Garcia, Cristina; Williams, Phil and Golding, Jon
Nanoscale Advances, 1(2) (pp. 807-816)

A comparison of the radiosensitisation ability of 22 different element metal oxide nanoparticles using clinical megavoltage X-rays (2019)
Guerreiro, Alexandra; Chatterton, Nicholas; Crabb, Eleanor and Golding, Jon
Cancer Nanotechnology, 10, Article 10

Photodynamic therapy and diagnosis: Principles and comparative aspects (2018-03)
Dobson, Jane; Fernandes de Queiroz, Genilson and Golding, Jon P.
Veterinary Journal, 233 (pp. 8-18)

Glycolysis inhibition improves photodynamic therapy response rates for equine sarcoids (2017-12)
Golding, J. P.; Kemp-Symonds, J. G. and Dobson, J. M.
Veterinary and Comparative Oncology, 15(4) (pp. 1543-1552)

Cancer-selective, single agent chemoradiosensitising gold nanoparticles (2017-07-10)
Grellet, Sophie; Tzelepi, Konstantina; Roskamp, Meike; Williams, Phil; Sharif, Aquila; Slade-Carter, Richard; Goldie, Peter; Whilde, Nicky; Smialek, Malgorzata A.; Mason, Nigel J. and Golding, Jon P.
PLoS ONE, 12, Article e0181103(7)

Gold nanoparticles for cancer radiotherapy: a review (2016-11-03)
Haume, Kaspar; Rosa, Soraia; Grellet, Sophie; Śmiałek, Małgorzata; Butterworth, Karl T.; Solov'yov, Andrey V.; Prise, Kevin M.; Golding, Jon and Mason, Nigel J.
Cancer Nanotechnology, 7(8)

Engineered neural tissue with aligned, differentiated adipose-derived stem cells promotes peripheral nerve regeneration across a critical sized defect in rat sciatic nerve. (2015-01-31)
Georgiou, Melanie; Golding, Jon P.; Loughlin, Alison J.; Kingham, Paul J. and Phillips, James B.
Biomaterials, 37 (pp. 242-251)

A 3D in vitro model reveals differences in the astrocyte response elicited by potential stem cell therapies for CNS injury. (2013-11-01)
East, Emma; Johns, Noémie; Georgiou, Melanie; Golding, Jon P.; Loughlin, A. Jane; Kingham, Paul J. and Phillips, James B.
Regenerative Medicine, 8(6) (pp. 739-746)

Engineered neural tissue for peripheral nerve repair (2013-10-01)
Georgiou, Melanie; Bunting, Stephen C. J.; Davies, Heather A.; Loughlin, Alison J.; Golding, Jonathan P. and Phillips, James B.
Biomaterials, 34(30) (pp. 7335-7343)

Targeting tumour energy metabolism potentiates the cytotoxicity of 5-aminolevulinic acid photodynamic therapy (2013-08-20)
Golding, J. P.; Wardhaugh, T.; Patrick, L.; Turner, M.; Phillips, J. B.; Bruce, J. I. and Kimani, S. G.
British Journal of Cancer, 109(4) (pp. 976-982)

Fully protected glycosylated zinc (II) phthalocyanine shows high uptake and photodynamic cytotoxicity in MCF-7 cancer cells (2013-01)
Kimani, Stanley; Shmigol, Tatiana; Hammond, Samantha; Phillips, James B.; Bruce, James I.; MacRobert, Alexander J.; Malakhov, Mikhail V. and Golding, Jon P.
Photochemistry and Photobiology, 89(1) (pp. 139-149)

Engineering an integrated cellular interface in three-dimensional hydrogel cultures permits monitoring of reciprocal astrocyte and neuronal responses (2012-07-11)
East, Emma; Golding, Jon P. and Phillips, James B.
Tissue Engineering Part C: Methods, 18(7) (pp. 526-536)

Regulation of PP2A activity by Mid1 controls cranial neural crest speed and gangliogenesis (2012-01)
Latta, Elizabeth J. and Golding, Jon P.
Mechanisms of Development, 128(11-12) (pp. 560-576)

Alignment of astrocytes increases neuronal growth in three-dimensional collagen gels and is maintained following plastic compression to form a spinal cord repair conduit (2010-10-01)
East, Emma; Blum de Oliveira, Daniela; Golding, Jon P. and Phillips, James B.
Tissue Engineering Part A, 16(10) (pp. 3173-3184)

Homing of stem cells to sites of inflammatory brain injury after intracerebral and intravenous administration: a longitudinal imaging study (2010-06)
Jackson, Johanna S.; Golding, Jon P.; Chapon, Catherine; Jones, William A. and Bhakoo, Kishore K.
Stem Cells Research & Therapy, 1(2) (pp. 1-17)

A versatile 3D culture model facilitates monitoring of astrocytes undergoing reactive gliosis (2009-10)
East, Emma; Golding, Jonathan P. and Phillips, James B.
Journal of Tissue Engineering and Regenerative Medicine, 3(8) (pp. 634-646)

Astrocyte alignment increases neurite outgrowth in a 3D cell culture model (2009)
East, Emma; Blum de Oliveira, Daniela; Golding, Jon and Phillips, James
Glia, 57(S13) (S159)

Developing a 3D culture model of CNS myelination (2009)
Loughlin, Jane; East, Emma; Golding, Jon and Phillips, James
Glia, 57(S13) (S119)

Skeletal muscle stem cells express anti-apoptotic ErbB receptors during activation from quiescence (2007-01-15)
Golding, Jon P; Calderbank, Emma; Partridge, Terence and Beauchamp, Jonathan
Experimental Cell Research, 313(2) (pp. 341-356)

Increased GFAP immunoreactivity by astrocytes in response to contact with dorsal root ganglia cells in a 3D culture model (2007)
East, Emma; Golding, Jon and Phillips, James
Neuron Glia Biology, 3(S1) (S119)

Mouse myotomes pairs exhibit left-right asymmetric expression of MLC3F and α-skeletal actin (2004-12)
Golding, Jon P.; Partridge, Terence A.; Beauchamp, Jonathan R.; King, Tim; Brown, Nigel A.; Gassmann, Martin and Zammit, Peter S.
Developmental Dynamics, 231(4) (pp. 795-800)

Heparin-binding EGF-like growth factor shows transient left-right asymmetrical expression in mouse myotome pairs (2004-11)
Golding, Jon P.; Tsoni, Stavroula; Dixon, Monica; Yee, Kathleen T.; Partridge, Terence A.; Beauchamp, Jonathan R.; Gassmann, Martin and Zammit, Peter S.
Gene Expression Patterns, 5(1) (pp. 3-9)

Myf5 expression in satellite cells and spindles in adult muscle is controlled by separate genetic elements (2004-09-15)
Zammit, Peter S.; Carvajal, Jaime J.; Golding, Jon P.; Morgan, Jennifer E.; Summerbell, Dennis; Zolnerciks, Joseph; Partridge, Terence A.; Rigby, Peter W.J. and Beauchamp, Jonathan R.
Developmental Biology, 273(2) (pp. 454-465)

Muscle satellite cells adopt divergent fates: a mechanism for self-renewal? (2004-08-02)
Zammit, Peter S.; Golding, Jon P.; Nagata, Yosuke; Hudon, Valerie; Partridge, Terence A. and Beauchamp, Jonathan R.
Journal of Cell Biology, 166(3) (pp. 347-357)

Roles of erbB4, rhombomere-specific, and rhombomere-independent cues in maintaining neural crest-free zones in the embryonic head (2004-02-15)
Golding, Jon P.; Sobieszczuk, Dorothy; Dixon, Monica; Coles, Edward; Christiansen, Jeff; Wilkinson, David and Gassmann, Martin
Developmental Biology, 266(2) (pp. 361-372)

ErbB4 signaling during breast and neural development: novel genetic models reveal unique ErbB4 activities (2003-11)
Jones, Frank E.; Golding, Jon P. and Gassmann, Martin
Cell Cycle, 2(6) (pp. 555-559)

Neural and mammary gland defects in ErbB4 knockout mice genetically rescued from embryonic lethality (2003-07-08)
Tidcombe, Hester; Jackson-Fisher, Amy; Mathers, Kathleen; Stern, David F.; Gassmann, Martin and Golding, Jon P.
Proceedings of the National Academy of Sciences of the United States of America, 100(14) (pp. 8281-8286)

Cues from neuroepithelium and surface ectoderm maintain neural crest-free regions within cranial mesenchyme of the developing chick (2002-03-01)
Golding, Jon P.; Dixon, Monica and Gassmann, Martin
Development, 129(5) (pp. 1095-1105)

Book chapters

New Research in Ionizing Radiation and Nanoparticles: The ARGENT Project (2016-12-16)
Bolsa Ferruz, M.; Ivošev, V.; Haume, K.; Ellis-Gibbings, L.; Traore, A.; Thakare, V.; Rosa, S.; de Vera, Pablo; Tran, V.-L.; Mika, A.; Boscolo, D.; Grellet, S.; Verkhovtsev, Alexey; Huber, Bernd A.; Butterworth, K. T.; Prise, K. M.; Currell, F. J.; Mason, Nigel J.; Golding, J.; Scifoni, E.; Garcia, Gustavo; Boschetti, F.; Lux, F.; Tillement, O.; Louis, C.; Stokbro, K.; Solov'yov, Andrey V. and Lacombe, S.
In: Solov'yov, Andrey V. ed. Nanoscale insights into ion-beam cancer therapy (pp. 379-434)
ISBN : 978-3-319-43030-0 | Publisher : Springer International Publishing | Published : Cham

Conference items

Cancer-selective toxicity of gold nanoparticles: effects of synthesis time and charge (2016-06-27)
Tzelepi, Nadia and Golding, Jon
In : CLINAM 2016 (26-29 Jun 2016, Basel, Switzerland)

Selective cancer cell toxicity and radiosensitization using different high atomic number nanoparticles (2016-06-02)
Grellet, S.; Kaas, S.; Crabb, E.; Allman, S. and Golding, J.
In : Nanotech France 2016 (1-3 Jun 2016, Paris, France)

Selective cancer cell toxicity and radiosensitization with gold nanoparticles (2015-10-19)
Grellet, Sophie; Williams, Phil and Golding, Jon
In : Advances in nanoparticulate carriers: Applications in diseases and infections (19-21 Oct 2015, Institut Pasteur, Paris)

Fabrication of an endoneurium using engineered neural tissue within a peripheral nerve repair conduit (2013-06)
Georgiou, Melanie; Golding, Jon; Loughlin, Jane and Phillips, James
In : Tissue Engineering and Regenerative Medicine International Society - EU Meeting (17-20 Jun 2013, Istanbul, Turkey)

A living replacement tissue for peripheral nerve that can enhance regeneration in vitro and in vivo (2012-09)
Georgiou, Melanie; Bunting, Stephen; Golding, Jon; Loughlin, Jane and Phillips, James
In : Tissue Engineering and Regenerative Medicine International Society - World Congress 2012 (5-8 Sep 2012, Vienna, Austria)

A nerve repair conduit containing differentiated adipose-derived stem cells within engineered neural tissue can support and guide neuronal growth in vitro and in vivo (2012-09)
Georgiou, Melanie; Kingham, Paul; Bunting, Stephen; Golding, Jon; Loughlin, Jane and Phillips, James
In : Tissue Engineering and Regenerative Medicine International Society - World Congress 2012 (5-8 Sep 2012, Vienna, Austria)

Astrocytes expressing GFP in 3D collagen gels provide an effective model for screening the glial response to potential CNS cell therapies (2012-07)
Johns, N.; East, E.; Golding, J. P.; Loughlin, A. J. and Phillips, J. B.
In : Annual meeting of the Tissue and Cell Engineering Society (TCES) (4-6 Jul 2012, Liverpool, UK)

Engineered neural tissue with aligned Schwann cells supports neuronal regeneration in vivo and can be assembled using differentiated adipose-derived stem cells (2012-07)
Georgiou, M.; Loughlin, J.; Golding, J. P.; Kingham, P. J. and Phillips, J. B.
In : Annual meeting of the Tissue and Cell Engineering Society (TCES) (4-6 Jul 2012, Liverpool, UK)

Engineered neural tissue with columns of aligned Schwann cell-like cells from differentiated adipose-derived stem cells can support and guide neuronal growth in vitro (2012-04)
Georgiou, Melanie; Kingham, Paul; Golding, Jon; Loughlin, Jane and Phillips, James
In : 3rd International Conference on Stem Cell Engineering "Designing Cellular Therapies" (29 Apr - 02 May 2012, Seattle, US)

Aligned Schwann cells within 3D tissue-like gels provide guidance to regenerating neurites (2011-09)
Georgiou, M.; East, E.; Loughlin, J.; Golding, J. and Phillips, J.
In : 10th European Meeting on Glial Cells in Health and Disease (13-17 Sep 2011, Prague, Czech Republic)

Aligned cellular and acellular collagen guidance substrates for peripheral nerve repair (2011-07)
Georgiou, Melanie; Loughlin, Jane; Golding, Jon and Phillips, James
In : Tissue and Cell Engineering Society (19-21 Jul 2011, Leeds)

A tissue engineered collagen conduit containing columns of aligned Schwann cells supports neuronal regeneration in vitro (2011)
Georgiou, M.; East, E.; Loughlin, A. J.; Golding, J. P. and Phillips, J. B.
In : 2011 PNS Biennial Meeting of the Peripheral Nerve Society (25-29 Jun 2011, Potomac, Maryland, US)

Schwann cells in collagen gels survive plastic compression and maintain their alignment: development of a cellular biomaterial for peripheral nerve repair (2010-07)
Georgiou, M.; East, E.; Loughlin, J.; Golding, J.P. and Phillips, J.B.
In : Tissue and Cell Engineering Society (28-30 Jul, Manchester, U.K.)

Development of an integrated collagen gel system for studying cellular interfaces following spinal cord injury (2010-07)
East, E.; Golding, J.P. and Phillips, J.B.
In : Tissue and Cell Engineering Society (28-30 Jul, Manchester, U.K.)

Plastic compression of aligned cellular collagen gels for nervous system repair (2010-06)
East, E.; Georgiou, M.; Loughlin, A.J.; Golding, J.P. and Phillips, J.B.
In : Tissue Engineering and Regenerative Medicine International Society - EU Meeting (13-17 Jun, Galway, Ireland)

Potentiation of AlPcS₂ mediated photodynamic therapy by energy metabolism inhibitors in human tumour cell lines (2009-09-05)
Kimani, Stanley; Phillips, James; Bruce, James; MacRobert, A. J. and Golding, Jon
In : 13th Congress of the European Society for Photobiology (5 Sep 2009, Warsaw, Poland)

Astrocyte alignment in 3D collagen gels increases neurite outgrowth; implications for improving spinal cord repair (2009-07)
East, E.; Blum de Oliveira, D.; Golding, J. P. and Phillips, J. B.
In : Tissue and Cell Engineering Society (8-10 Jul 2009, Glasgow) (pp. 13-13)

Monitoring reactive gliosis in 3-dimensional astrocyte cultures; a model of spinal cord damage (2008-11-19)
Phillips, James; East, Emma and Golding, Jon
In : Neuroscience 2008: 38th annual meeting of the Society for Neuroscience (15-19 Nov 2008, Washington, DC)

Glucosamine improves the efficiency of photodynamic therapy (PDT) (2008-10-07)
Kimani, Stanley; Phillips, James; Bruce, James; MacRobert, A. J and Golding, Jon
In : Photodynamic Therapy and Photodiagnosis in Clinical Practice (7-11 Oct 2008, Brixen, Italy)

Modelling the injured spinal cord using 3-dimensional cell cultures; strategies for improving tissue engineered repair (2008)
East, E.; Golding, J. P. and Phillips, J. B.
In : Annual meeting of the Tissue and Cell Engineering Society (TCES) (2-4 Jul 2008, University of Nottingham, UK) (p 51)

Midline1 and the development of the cranial peripheral nervous system (2008)
Latta, Elizabeth; Saffrey, Jill and Golding, Jon
In : Joint meeting of the British and Spanish Developmental Biology Societies (24-27 Sep 2008, Seville, Spain)

Modelling of the injured spinal cord using 3-dimensional cell cultures; strategies for improving tissue engineered repair (2007-11-06)
East, E.; Golding, J.P. and Phillips, J.B.
In : Society for Neuroscience (3-7 Nov 2007, San Diego, California)

An in vivo model of epidermal neural crest cell migration after implantation into the brain (2007-11-04)
Jackson, J.S.; Golding, J.; Chapon, C.; Jones, W. and Bhakoo, K.K.
In : Society for Neuroscience (3-7 Nov 2007, San Diego, California)

Development of a 3-dimensional in vitro model to study reactive gliosis following nervous system injury (2007-07)
East, Emma; Golding, Jon and Phillips, James
In : Tissue Engineering - EU Chapter meeting 2007 (4-7 Sep 2007, London, UK) (pp. 1668-1778)

Maintaining the regenerative compartment of adult skeletal muscle (2005-05-09)
Beauchamp, J.R.; Zammit, P.S.; Nagata, Y.; Golding, J. and Partridge, T.A.
In : Congress of Myology (09-13 May 2005, Nantes, France)

Not all activated satellite cell progeny commit to differentiation (2003-09)
Golding, J.P.; Beauchamp, J.R.; Partridge, T.A. and Zammit, P.S.
In : 32nd European Muscle Conference (7-10 Sep 2003, Montpellier, France)

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Professional biography

Research interests

Teaching interests

Impact and engagement

External collaborations

International links

Research Activity

Research groups

Externally funded projects

Development of H19-targeting antisense oligonucleotides for DIPG therapy

Improving the efficiency and targeting of radiosensitisers

Mechanisms of nanoparticle toxicity

Publications

Journal articles

Book chapters

Conference items

Web links

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