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Professor Nacho Romero

Profile summary

  • Central Academic Staff
  • Professor in Cellular Neuroscience
  • Faculty of Science, Technology, Engineering & Mathematics
  • School of Life, Health & Chemical Sciences
  • nacho.romero

Professional biography

I am a cell biologist with research and teaching interests in blood-brain barrier physiology and pathology and in neuroimmunology.

Research interests

Research interests:

            My research interests focus on the function of cerebral blood vessels in health and disease. In particular, I am interested in the pathogenic mechanisms of blood-brain barrier (BBB) dysfunction in disorders of the central nervous system (CNS) such as multiple sclerosis (MS) and Alzheimer’s disease (AD). I have currently focused my research strategy in identifying the molecular mechanisms mediating abnormal increases in BBB permeability and in leukocyte migration, which ultimately contribute to neuronal death. My research has applications not only as a means to identify potential therapeutic targets for CNS pathologies but also to provide a basis to enhance drug delivery to the CNS. In order to address these questions, my research group uses cell and molecular techniques applied to cultured human brain endothelium and astrocytes and has collaborations in place to correlate our results with those obtained using in vivo animal models of disease. In collaboration with a group in Paris, we have immortalized primary human brain endothelial cells isolated from a patient undergoing temporal lobe resection by transduction with lentiviral vectors incorporating human telomerase or SV40 T antigen. This cell line, termed hCMEC/D3, demonstrates BBB characteristics, including expression of tight junctional proteins and the capacity to actively exclude drugs and is therefore an effective in vitro human BBB model. hCMEC/D3 cells have been distributed to over 120 labs worldwide as a tool to study different aspects of BBB physiology and pathology and generated to date over 40 publications. The following research areas will generate defined research outputs in the form of high-impact research articles and preliminary data for future grant applications as stated below.

            1) Transcriptional and post-transcriptional regulation of human brain endothelial cell phenotype under normal and inflammatory conditions and in ageing: The general aim of this project is to establish a link between the inflammatory signals from the CNS (either cell or viral derived) and the transcriptional environment that regulate expression of three tight junctional proteins (occludin, claudin-5 and ZO-1) and two drug efflux transporters (Pgp and BCRP) in brain endothelium. To do so, we are in the process of determining the promoter regions that are important for occludin, claudin-5 and ZO-1, Pgp and BCRP expression in human brain endothelial cell lines and the transcriptional environment (transcription factors, histone modifications) that modulates expression under different inflammatory conditions such as cytokine and chemokine stimulation (e.g. TNFa, IL1b, CXCL10) and viral-derived products (HIV, HTLV). More recently, my research group has been focused on the role of microRNAs in the regulation of BBB permeability and leukocyte transmigration under inflammatory conditions. We have identified a microRNA signature consisting of approximately 50 microRNAs deregulated in neuroinflammation that ensures low BBB permeability and immunoquiescence under basal conditions. The information from this project and from the project above will provide the basis to selectively manipulate tight junction permeability at the BBB to drugs.

PhD students: Hadassah Sade (2004-2008; 2nd supervisor); Leon Tai (2005-2009); Alejandro Lopez (2008-2012); Camilla Cerutti (2009-2013)

Postdoctoral Fellows: Karen Logan (2002-2009); Dongsheng Wu (2011-present); Cheungfun Wang (2013-present)

Funded by: the Leverhulme Trust, the DANA foundation, Medical Research Council and the Multiple Sclerosis Society, BBSRC

Collaborators: Prof David Male and Dr Mark Hirst (Open University), Dr Basil Sharrack, Dr Paul Heath and Dr Steven Wharton (University of Sheffield), Prof David Baker and Dr Greg Michael (QMUL).

            2) Refinement of an in vitro human blood-brain barrier model: We intend to improve our current BBB model by two approaches: 1) Using a dynamic flow in vitro BBB model to mimic more closely the in vivo phenotype to study leukocyte adhesion and migration to brain endothelium. A major aim of our research is to adapt this model to investigate delivery of therapies to the CNS. 2) Establishing a three-dimensional model of the BBB to study astrocyte-endothelial cell interactions in collagen gel cultures.

PhD students: Sreekanth Reddy (2008-2012; 2nd supervisor)

Funded by:  The Open University Research Development Fund.

Collaborators: Dr Pierre-Olivier Couraud (ICGM, Paris), Dr Babette Weksler (Cornell University, New York), Dr Jane Preston (KCL), Dr James Phillips (Open University).

            3) Drug delivery to the CNS: This is a new project starting in 2012 involving a PhD student and a PDRA in which we propose to use aptamers that specifically bind to brain endothelial cells as drug delivery vehicles to produce an entirely novel system for effective delivery of small molecules to the CNS. Aptamers have the added advantages over antibodies and/or peptides of being more chemically robust, less expensive to generate and more easily modified. Therefore, nucleic acid aptamers specific for surface moieties on brain endothelial cells that are subsequently internalized and transcytosed could prove to be novel delivery CNS transport vectors.  We propose to identify brain endothelial-specific nucleic acid aptamers using SELEX (Systematic Evolution of Ligands by EXponential enrichment) on either whole cultured cells or recombinant proteins known to be enriched on the cell surface at the BBB and to further select aptamers that are internalized and/or transcytosed by human and rat in vitro BBB models.

PhD students: Mohammad Daas (2013-present); Radka Gromnicova (2013-present; 2nd supervisor)

Funded by: MedImmune.

Collaborators: Prof David Male and Dr Carol Midgley (Open University).

Recent Research Grants:

 

 

2013-2016

BBSRC

Role of microRNAs in ageing at the blood-brain barrier. £386,000. Romero (PI), Saffrey (OU); Wharton, Heath (Sheffield)

2013-2016

MedImmune

Development of a drug delivery system to the CNS using brain endothelial-specific non-antibody binding domains as transport carriers. £75,000. Romero (PI)

2013-2016

OU Res Dev Fund/NHS charter studentship

Molecular mechanisms mediating idiopathic intracranial hypertension. £45,385. Romero (PI) (OU), Preston (KCL), Sharrack (Sheffield)

2011-2014

Multiple Sclerosis Society of Great Britain and Northern Ireland

MicroRNAs in the cerebral vasculature and multiple sclerosis. £205,122. Romero (PI), Hirst, Male (OU), Sharrack (Sheffield), Baker, Michael (QMUL)

2009-2012

OU Res Dev Fund/NHS charter studentship

Role of microRNAs in regulation of leukocyte transmigration in neuroinflammation. £55,000 Romero (PI) (OU), Sharrack (Sheffield)

2009

Multiple Sclerosis Society of Great Britain and Northern Ireland

Expression profile of microRNAs by human brain endothelium in neuroinflammation: implications for blood-brain barrier breakdown in multiple sclerosis. £25,677. Romero (PI), Male, Hirst (OU) and Sharrack (Sheffield).

2008-2011

OU Res Dev Fund/NHS charter studentship

Role of microRNAs in regulation of BBB permeability in neuroinflammation. £55,000 Romero (PI) (OU), Sharrack (Sheffield)

2006-2009

DANA Foundation

Determining the transcriptional environment that suppresses expression of tight junctional proteins at the blood-brain barrier in neuroinflammation. $200,000. Romero (PI) and Male (OU)

 

Teaching interests

Overview: Open University teaching is based upon distance learning, with students studying core teaching materials designed, produced and led by academics. Our modules are studied for both standard undergraduate purposes and for CPD by professional scientists across the world. Our module materials are produced by teams of academics and production media specialists and teaching is directly provided in small groups by our specialised teams of associated lecturers. Modules are taken by a number of students ranging from ~100 (e.g. practical modules) to ~1,500 (e.g. biological psychology) every presentation. Assessment is in the form of tutor- (TMA) or computer-marked (CMA) assessments and examinable components (ECA). My main teaching area reflects my research field of cell and molecular neurobiology.

Roles in modules I have contributed to

Module Team Chair: Level 3 The molecular basis of human disease (15 points) (2009-2012) and Level 2 Human Biology (30 points) (2004-2005): As a module chair I worked on overseeing the presentation of the which includes the delivery of assessment to students, chairing Exam Boards, Standardisation and Awards meetings and organizing script marking.

Author of distance teaching material (text and activities) and assessment: Level 3 Infectious Disease, author 2 chapters and computer-marked assessment; Level 1 Understanding Cancers, Author 5 chapters, Web-based CD-ROM activities, and computer-marked continuous and examinable assessment; Level 3 The molecular basis of human disease, author 3 chapters CD-ROM based Experimental Investigation and Activities; Level 2 Biological psychology: Exploring the Brain, author 2 chapters, Web-based ‘Exploring the Brain’ CD-ROM, tutor-marked continuous and examinable assessment; Level 3 Molecular and Cell Biology, author 2 chapters, CD-ROM based Activities and tutor-marked continuous and examinable assessment.

Design of Laboratory Practical Experimentation: Level 3 The molecular basis of human disease: (15 points) (2006-2012). A laboratory based module studied by 120 students per year, focussed on the relationship between human genetic variation and susceptibility to HIV1 infection. Practical teaching covered area of PCR and western blot analysis.

Design of Virtual Experimentation: Level 2 Practical Science Drug metabolism and genetic variation (30 points) (2010-present); Level 3 Plants, Pigments and Light (15 points) (2004-2005); Level 3 Fat – the physiology of adipose tissue (15 points) (2004-2005); Level 3 The molecular basis of human disease: (15 points) (2006-2012).

Extra-curricular Distance Teaching: Academic Editor of Communicable Diseases module and workshop facilitator for the Open University’s Health Education and Training (HEAT) Programme in collaboration with the Ethiopian Federal Ministry of Health (2011), aimed at training frontline healthcare workers in Sub-Saharan Africa.

Research Activity

Research groups

NameTypeParent Unit
Biomedical Research Network (BRN)NetworkFaculty of Science

 

Externally funded projects

Investigation into the molecular mechanisms underlying idiopathic intracranial hypertension (IIH)

RoleStart dateEnd dateFunding source
Lead01/Oct/201630/Sep/2019Sheffield Teaching Hospitals
Investigation into the molecular mechanisms underlying idiopathic intracranial hypertension Idiopathic intracranial hypertension (IIH) is a neurological condition characterized by increased intracranial pressure in the absence of a tumour or other diseases. IIH affects primarily pre-menopausal obese women but the contribution of reproductive hormones and/or obesity to the pathogenesis of this disease is unknown. Indeed, as its name implies, its aetiology remains elusive although a possible role for inflammatory mediators has been suggested. From a physiological point of view, raised intracranial pressure may be the result of increased cerebrospinal fluid (CSF) secretion, decreased CSF drainage or a combination of both factors. The objectives for this project are: Year 1. To determine transcriptomic and microRNA changes in choroid plexus of female control rats and rats fed a high-fat diet and in young and old female rats. Years 2 and 3. First, to establish an in vitro rat model of the blood-CSF barrier and, second, to select candidates from the transcriptomic/microRNA analysis that are differentially regulated and investigate their effects on the secretory and transport activity of rat and/or human choroid plexus epithelium (both cell lines and primary cultures)

Long non-coding RNAs implicated in neuroendocrine prostate cancer initiation and progression: novel therapeutic targets for an incurable disease.

RoleStart dateEnd dateFunding source
Co-investigator01/Sep/201631/Aug/2019Cancer Research UK (CRUK)
Neuroendocrine prostate cancer (NEPC) is the most aggressive prostate cancer type. This disease is resistant to all available therapies, highly metastatic and rapidly fatal. We believe that the elucidation of NEPC-driving pathways could pave the way for the development of effective therapies. Long non-coding RNAs (lncRNAs) are a vast and mostly uncharted region of the human transcriptome. Despite their emerging role in cancer biology, no study has assessed the relevance of lncRNAs in NEPC development. Our collaborator Dr. Wang (BC Cancer Agency) has developed a unique collection of patient-derived prostate cancer models. This collection includes the first-in-field model of transdifferentiation from prostatic adenocarcinoma to NEPC. Aims. We propose to study the role of lncRNAs in NEPC development. The objectives of this study are to determine: 1) whether transcriptomic profiles of lncRNAs in PCa pre-clinical models can elucidate the mechanisms of NEPC development. 2) if selected lncRNAs are promising therapeutic targets for NEPC. Accordingly, our specific aims will be: AIM 1: Identification, functional and clinical characterization of NEPC-driving lncRNAs. AIM 2: Development of innovative therapeutic strategies to prevent NEPC development. At the end of this project, we hope to shed new light on an incurable disease, and to identify viable therapeutic targets, which will be investigated in future translational and clinical studies.

Investigation Into The Molecular Mechanisms Underlying Idiopathic Intracranial Hypertension (IIH) - Four Months Stipend Payment extension

RoleStart dateEnd dateFunding source
Lead01/Feb/201631/May/2016Sheffield Teaching Hospitals
IIH is a neurological condition characterized by increased intracranial pressure in the absence of a tumor or other diseases but whose cause remains elusive. A possible role for leptin and inflammatory mediators such as CCL2 has been suggested. The objectives for this project are: 1. To determine, the effect of these inflammatory mediators on cerebrospinal fluid (CSF) secretion and drainage 2. To determine the role of sex and high-fat diet on CSF secretion and drainage. 3. to establish an in vitro human model of the blood-CSF barrier and to determine , using transcriptomic analysis, the effect of inflammatory mediators on gene profile of cultures of human choroid plexus epithelium . These results may help identify potential therapeutic molecular targets for IIH

Role of microRNAs in endothelial-derived extracellular vesicles on leukocyte migration across the blood-brain barrier

RoleStart dateEnd dateFunding source
Lead01/Oct/201530/Sep/2018Sheffield Teaching Hospitals
Intercellular communication between immune cells and tissue-resident cells is essential to coordinate an effective immune response and involves both cell-contact dependent and independent processes that ensure the transfer of information between bystander and distant cells. There is a rapidly growing body of evidence on the pivotal role of extracellular vesicles (EVs) in cell communication and these structures are emerging as important mediators for immune modulation upon delivery of their molecular cargo. In the last decade, EVs have been shown to be efficient carriers of genetic information, including microRNAs, that can be transferred between cells and regulate gene expression and function on the recipient cell. However, little is known about regulation of cellular function by EVs at the blood-brain barrier (BBB), the main route of entry of immune cells into the central nervous system (CNS), in pathological conditions. We have recently shown that EVs isolated from MS patients induce blood-brain barrier dysfunction, characterised by leakiness of the barrier, in a human culture model (1) and that the microRNA profile of brain endothelium undergoes profound changes in inflammation (2,3). Thus, the proposed project will investigate the role of microRNAs secreted in EVs in activation of brain endothelium and subsequent leukocyte migration. In year 1, the miroRNA profile of EVs released by cultured human brain endothelium in inflammatory conditions and in plasma of MS patients will be determined. In year 2, the effects of endothelial-derived and MS plasma-derived EVs on leukocyte adhesion, both leukocyte cell lines and peripheral blood mononuclear cells, under flow will be investigated. In year 3, specific microRNAs will be knocked-down in cultured human brain endothelium prior to EV collection and subsequent effects on leukocyte adhesion will be investigated

BtRAIN (Brain Barriers training) - To understand the brain barriers

RoleStart dateEnd dateFunding source
Lead01/Oct/201530/Sep/2019EC (European Commission): FP(inc.Horizon2020, H2020, ERC)
The general aim of the current proposal is to the function of the neurovascular unit across species and the impact of several conditions (development, inflammation, ageing, exposure to drugs)on the function of the blood-brain barriers. The aims are: 1. Prediciting brain barrier function using in vitro BBB models 2. Brain barriers signature in vertrebrates 3. Brain barriers as therapeutic and diagnostic target The OU will lead the project within the BtRAIN network focused on age-associated changes in gene and microRNA expression at the blood-brain barrier. See the network webpage http://www.btrain-2020.eu/

Publications

Age-associated changes in the blood brain barrier: Comparative studies in human and mouse (2017)
Goodall, Emily F.; Wang, Chunfang; Simpson, Julie E.; Baker, David J.; Drew, David R.; Heath, Paul R.; Saffrey, M. Jill; Romero, Ignacio A. and Wharton, Stephen B.
Neuropathology and Applied Neurobiology ((Early Access))
Early alterations of Hedgehog signaling pathway in vascular endothelial cells after peripheral nerve injury elicit blood-nerve barrier disruption, nerve inflammation, and neuropathic pain development (2016-04)
Moreau, Nathan; Mauborgne, Annie; Bourgoin, Sylvie; Couraud, Pierre-Olivier; Romero, Ignacio A.; Weksler, Babette B.; Villanueva, Luis; Pohl, Michel and Boucher, Yves
PAIN, 157(4) (pp. 827-839)
Localisation and mobility of glucose-coated gold nanoparticles within the brain (2016-03)
Gromnicova, Radka; Yilmaz, Canan Ugur; Orhan, Nurcan; Kaya, Mehmet; Davies, Heather; Williams, Phil; Romero, Ignacio A.; Sharrack, Basil and Male, David
Nanomedicine, 11(6) (pp. 617-625)
A three-dimensional model of the human blood-brain barrier to analyse the transport of nanoparticles and astrocyte/endothelial interactions (2016-01-21)
Reddy, P; Gromnicova, Radka; Davies, Heather; Phillips, James; Romero, Ignacio A and Male, David
F1000Research, 4, Article 1279
Transport of Gold Nanoparticles by Vascular Endothelium from Different Human Tissues (2016)
Gromnicova, Radka; Kaya, Mehmet; Romero, Ignacio A.; Williams, Phil; Satchell, Simon; Sharrack, Basil and Male, David
PLoS ONE, 11, Article e0161610(8)
Insulin and IGF1 signalling pathways in human astrocytes in vitro and in vivo; characterisation, subcellular localisation and modulation of the receptors. (2015-12)
Garwood, Claire J.; Ratcliffe, Laura E.; Morgan, Sarah V.; Simpson, Julie E.; Owens, Helen; Vazquez-Villaseñor, Irina; Heath, Paul R.; Romero, Ignacio A.; Ince, Paul G. and Wharton, Stephen B.
Molecular Brain, 8, Article 51(1)
Inflammatory response of endothelial cells to a human endogenous retrovirus associated with multiple sclerosis is mediated by TLR4. (2015-11-01)
Duperray, Alain; Barbe, Delphin; Raguenez, Gilda; Weksler, Babette B.; Romero, Ignacio A.; Couraud, Pierre-Olivier; Perron, Hervé and Marche, Patrice N.
International Immunology, 27(11) (pp. 545-553)
Serotype O18 avian pathogenic and neonatal meningitis Escherichia coli strains employ similar pathogenic strategies for the onset of meningitis. (2015-11)
Krishnan, Subramanian; Chang, Alexander C.; Hodges, Jacqueline; Couraud, Pierre-Olivier; Romero, Ignacio A.; Weksler, Babette; Nicholson, Bryon A.; Nolan, Lisa K. and Prasadarao, Nemani V.
Virulence, 6(8) (pp. 777-786)
Zinc-imidazolate polymers (ZIPs) as a potential carrier to brain capillary endothelial cells (2015-10-26)
Chiacchia, M.; Cerutti, C.; Gromnicova, R.; Rietdorf, K.; Romero, I.A. and Bradshaw, D.
Journal of Materials Chemistry B, 3(46) (pp. 9053-9059)
Characterizing the pathotype of neonatal meningitis causing Escherichia coli (NMEC) (2015-10-14)
Wijetunge, D. S. S.; Gongati, S. ; DebRoy, C. ; Kim, K. S. ; Couraud, P. O. ; Romero, I. A.; Weksler, B. and Kariyawasam, S.
BMC Microbiology, 15, Article 211
Differential permissivity of human cerebrovascular endothelial cells to enterovirus infection and specificities of enterovirus 71 in crossing an in vitro model of human blood brain barrier (2015-07)
Volle, Romain; Archimbaud, Christine; Couraud, Pierre-Olivier; Romero, Ignacio A.; Weksler, Babette; Mirand, Audrey; Pereira, Bruno; Henquell, Cécile; Peigue-Lafeuille, Hélène and Bailly, Jean-Luc
Journal of General Virology, 96 (pp. 1682-1695)
Brain endothelial miR-146a negatively modulates T-cell adhesion through repressing multiple targets to inhibit NF-κB activation. (2015-03)
Wu, Dongsheng; Cerutti, Camilla; Lopez-Ramirez, Alejandro; Pryce, Gareth; King-Robson, Josh; Simpson, Julie E.; van der Pol, Susanne M. A.; Hirst, Mark C.; de Vries, Helga E.; Sharrack, Basil; Baker, David; Male, David K.; Michael, Gregory J. and Romero, Ignacio A
Journal of Cerebral Blood Flow & Metabolism, 35(3) (pp. 412-23)
Permeability of PEGylated immunoarsonoliposomes through in vitro blood brain barrier: medulloblastoma co-culture models for brain tumor therapy (2015-03)
Al-Shehri, Abdulghani; Favretto, Marco E.; Ioannou, Panayiotis V.; Romero, Ignacio A.; Couraud, Pierre-Olivier; Weksler, Babette Barbash; Parker, Terry L. and Kallinteri, Paraskevi
Pharmaceutical research, 32(3) (pp. 1072-83)
The blood-brain barrier in multiple sclerosis: microRNAs as key regulators (2015)
Kamphuis, W. W.; Derada Troletti, C.; Reijerkerk, A.; Romero, I. A. and de Vries, H. E.
CNS & Neurological Disorders Drug Targets, 14(2) (pp. 157-67)
Protective effect of the multitarget compound DPH-4 on human SSAO/VAP-1-expressing hCMEC/D3 cells under oxygen-glucose deprivation conditions, an in vitro experimental model of cerebral ischemia. (2015)
Sun, P.; Esteban, G.; Inokuchi, T.; Marco-Contelles, J.; Weksler, B. B.; Romero, I. A.; Couraud, P. O.; Unzeta, M. and Solé, M.
British Journal of Pharmacology ((Early view))
The poorly membrane permeable antipsychotic drugs amisulpride and sulpiride are substrates of the organic cation transporters from the SLC22 family. (2014-11)
Dos Santos Pereira, Joao N.; Tadjerpisheh, Sina; Abu Abed, Manar; Saadatmand, Ali R.; Weksler, Babette; Romero, Ignacio A.; Couraud, Pierre-Olivier; Brockmöller, Jürgen and Tzvetkov, Mladen V.
AAPS Journal, 16(6) (pp. 1247-1258)
Microparticles in multiple sclerosis and clinically isolated syndrome: effect on endothelial barrier function. (2014-09-22)
Marcos-Ramiro, Beatriz; Oliva Nacarino, Pedro; Serrano-Pertierra, Esther; Blanco-Gelaz, Miguel Ángel; Weksler, Babette B.; Romero, Ignacio A.; Couraud, Pierre O.; Tuñón, Alberto; López-Larrea, Carlos; Millán, Jaime and Cernuda-Morollón, Eva
BMC Neuroscience, 15, Article 110
Synthesis and in vitro evaluation of BBB permeability, tumor cell uptake, and cytotoxicity of a series of carboranylporphyrin conjugates. (2014-08-14)
Bhupathiraju, N. V. S. Dinesh K.; Hu, Xiaoke; Zhou, Zehua; Fronczek, Frank R.; Couraud, Pierre-Olivier; Romero, Ignacio A.; Weksler, Babette and Vicente, M. Graça H.
Journal of Medicinal Chemistry, 57(15) (pp. 6718-28)
MicroRNA-155 negatively affects blood-brain barrier function during neuroinflammation. (2014-06)
Lopez-Ramirez, Miguel Alejandro; Wu, Dongsheng; Pryce, Gareth; Simpson, Julie E.; Reijerkerk, Arie; King-Robson, Josh; Kay, Oliver; de Vries, Helga E.; Hirst, Mark C.; Sharrack, Basil; Baker, David; Male, David Kingsley; Michael, Gregory J. and Romero, Ignacio Andres
FASEB Journal, 28(6) (pp. 2551-2565)
CXCL1/CXCL8 (GROα/IL-8) in human diabetic ketoacidosis plasma facilitates leukocyte recruitment to cerebrovascular endothelium in vitro (2014-05-01)
Omatsu, Tatsushi; Cepinskas, Gediminas; Clarson, Cheril; Patterson, Eric K.; Alharfi, Ibrahim M.; Summers, Kelly; Couraud, Pierre-Olivier; Romero, Ignacio A.; Weksler, Babette and Fraser, Douglas D.
American Journal of Physiology. Endocrinology and Metabolism, 306(9) (E1077-E1084)
Paracrine signalling of inflammatory cytokines from an in vitro blood brain barrier model upon exposure to polymeric nanoparticles. (2014-03-07)
Raghnaill, Michelle Nic; Bramini, Mattia; Ye, Dong; Couraud, Pierre-Olivier; Romero, Ignacio A.; Weksler, Babette; Aberg, Christoffer; Salvati, Anna; Lynch, Iseult and Dawson, Kenneth A.
The Analyst, 139(5) (pp. 923-930)
Differences in amyloid-β clearance across mouse and human blood-brain barrier models: kinetic analysis and mechanistic modeling. (2014-01-24)
Qosa, Hisham; Abuasal, Bilal S.; Romero, Ignacio A.; Weksler, Babette; Couraud, Pierre-Oliver; Keller, Jeffrey N. and Kaddoumi, Amal
Neuropharmacology, 79 (pp. 668-678)
Drug-induced trafficking of p-glycoprotein in human brain capillary endothelial cells as demonstrated by exposure to mitomycin C. (2014)
Noack, Andreas; Noack, Sandra; Hoffmann, Andrea; Maalouf, Katia; Buettner, Manuela; Couraud, Pierre-Olivier; Romero, Ignacio A.; Weksler, Babette; Alms, Dana; Römermann, Kerstin; Naim, Hassan Y. and Löscher, Wolfgang
PloS one, 9, Article e88154(2)
Oxidative and pro-inflammatory impact of regular and denicotinized cigarettes on blood brain barrier endothelial cells: is smoking reduced or nicotine-free products really safe? (2014)
Naik, Pooja; Fofaria, Neel; Prasad, Shikha; Sajja, Ravi K.; Weksler, Babette; Couraud, Pierre-Olivier; Romero, Ignacio A. and Cucullo, Luca
BMC Neuroscience, 15, Article 51
Glucose-coated gold nanoparticles transfer across human brain endothelium and enter astrocytes in vitro (2013-12-05)
Gromnicova, Radka; Davies, Heather A.; Sreekanthreddy, Pedagannganagari; Romero, Ignacio A.; Lund, Torben; Roitt, Ivan M.; Phillips, James B. and Male, David K.
PLoS ONE, 8, Article e81043(12)
Cytokine-induced changes in the gene expression profile of a human cerebral microvascular endothelial cell-line, hCMEC/D3. (2013-09-19)
Lopez-Ramirez, Miguel Alejandro; Male, David Kingsley; Wang, Chunfang; Sharrack, Basil; Wu, Dongsheng and Romero, Ignacio Andres
Fluids and Barriers of the CNS, 10, Article 27(1)
A recombinant inhibitory isoform of vascular endothelial growth factor164/165 aggravates ischemic brain damage in a mouse model of focal cerebral ischemia. (2013-09)
Chaitanya, Ganta V.; Cromer, Walter E.; Parker, Courtney P.; Couraud, Pierre O.; Romero, Ignacio A; Weksler, Babette; Mathis, J. Michael; Minagar, Alireza and Alexander, J. Steven
American Journal of Pathology, 183(3) (pp. 1010-1024)
Somatostatin preserved blood brain barrier against cytokine induced alterations: possible role in multiple sclerosis. (2013-08-15)
Basivireddy, Jayasree; Somvanshi, Rishi K.; Romero, Ignacio A; Weksler, Babette B.; Couraud, Pierre-Olivier; Oger, Joel and Kumar, Ujendra
Biochemical Pharmacology, 86(4) (pp. 497-507)
Temporal profile of matrix metalloproteinases and their inhibitors in a human endothelial cell culture model of cerebral ischemia. (2013-07)
Reuter, B.; Rodemer, C.; Grudzenski, S.; Couraud, P.-O.; Weksler, B.; Romero, Ignacio A; Meairs, S.; Bugert, P.; Hennerici, M. G and Fatar, M.
Cerebrovascular Diseases, 35(6) (pp. 514-520)
A multi-system approach assessing the interaction of anticonvulsants with P-gp. (2013-05-31)
Dickens, David; Yusof, Siti R.; Abbott, N. Joan; Weksler, Babette; Romero, Ignacio A; Couraud, Pierre-Olivier; Alfirevic, Ana; Pirmohamed, Munir and Owen, Andrew
PLoS ONE, 8, Article e64854(5)
Targeting endothelial CD146 attenuates neuroinflammation by limiting lymphocyte extravasation to the CNS. (2013-04-18)
Duan, Hongxia; Xing, Shu; Luo, Yongting; Feng, Liqun; Gramaglia, Irene; Zhang, Ying; Lu, Di; Zeng, Qiqun; Fan, Kelong; Feng, Jing; Yang, Dongling; Qin, Zhihai; Couraud, Pierre-Olivier; Romero, Ignacio A; Weksler, Babette and Yan, Xiyun
Scientific reports, 3, Article 1687
MicroRNAs regulate human brain endothelial cell-barrier function in inflammation: implications for multiple sclerosis. (2013-04-17)
Reijerkerk, Arie; Lopez-Ramirez, M. Alejandro; van Het Hof, Bert; Drexhage, Joost A. R.; Kamphuis, Wouter W.; Kooij, Gijs; Vos, Joost B.; van der Pouw Kraan, Tineke C. T. M.; van Zonneveld, Anton J.; Horrevoets, Anton J.; Prat, Alexandre; Romero, Ignacio A and de Vries, Helga E.
The Journal of Neuroscience , 33(16) (pp. 6857-6863)
High-density lipoproteins limit neutrophil-induced damage to the blood–brain barrier in vitro (2013-04)
Bao Dang, Quoc; Lapergue, Bertrand; Tran-Dinh, Alexy; Diallo, Devy; Moreno, Juan-Antonio; Mazighi, Mikael; Romero, Ignacio A.; Weksler, Babette; Michel, Jean-Baptiste; Amarenco, Pierre and Meilhac, Olivier
Journal of Cerebral Blood Flow & Metabolism, 33(4) (pp. 575-582)
BBB ON CHIP: microfluidic platform to mechanically and biochemically modulate blood-brain barrier function (2013-02-01)
Griep, L. M.; Wolbers, F.; de Wagenaar, B.; ter Braak, P. M.; Weksler, B. B.; Romero, I. A; Couraud, P. O.; Vermes, I.; van der Meer, A. D. and van den Berg, A.
Biomedical Microdevices, 15(1) (pp. 145-150)
Identification of an essential endogenous regulator of blood-brain barrier integrity, and its pathological and therapeutic implications (2013-01-15)
Cristante, Enrico; McArthur, Simon; Mauro, Claudio; Maggioli, Elisa; Romero, Ignacio A.; Wylezinska-Arridge, Marzena; Couraud, Pierre O.; Lopez-Tremoleda, Jordi; Christian, Helen C.; Weksler, Babette B.; Malaspina, Andrea and Solito, Egle
Proceedings of the National Academy of Sciences of the United States of America, 110(3) (pp. 832-841)
Immortalized human cerebral microvascular endothelial cells maintain the properties of primary cells in an in vitro model of immune migration across the blood brain barrier (2013-01-15)
Daniels, Brian P.; Cruz-Orengo, Lillian; Pasieka, Tracy Jo; Couraud, Pierre-Olivier; Romero, Ignacio A.; Weksler, Babette; Cooper, John A.; Doering, Tamara L. and Klein, Robyn S.
Journal of Neuroscience Methods, 212(1) (pp. 173-179)
The angiogenic gene profile of circulating endothelial progenitor cells from ischemic stroke patients. (2013)
Navarro-Sobrino, Míriam; Hernández-Guillamon, Mar; Fernandez-Cadenas, Israel; Ribó, Marc; Romero, Ignacio A; Couraud, Pierre-Olivier; Weksler, Babette Barbash; Montaner, Joan and Rosell, Anna
Vascular Cell, 5(3) (p 3)
The hCMEC/D3 cell line as a model of the human blood brain barrier (2013)
Weksler, Babette; Romero, Ignacio A. and Couraud, Pierre-Olivier
Fluids and barriers of the CNS, 10, Article 16(1)
The association of statins plus LDL receptor-targeted liposome-encapsulated doxorubicin increases the in vitro drug delivery across blood-brain barrier cells (2012-12)
Pinzón-Daza, M. L.; Garzón, R.; Couraud, P. O.; Romero, I. A.; Weksler, B.; Ghigo, D.; Bosia, A. and Riganti, C.
British Journal of Pharmacology, 167(7) (pp. 1431-1447)
Cell-penetrating anti-GFAP VHH and corresponding fluorescent fusion protein VHH-GFP spontaneously cross the blood-brain barrier and specifically recognize astrocytes: application to brain imaging (2012-10-22)
Li, Tengfei; Bourgeois, Jean-Pierre; Celli, Susanna; Glacial, Fabienne; Le Sourd, Anne-Marie; Mecheri, Salah; Weksler, Babette; Romero, Ignacio; Couraud, Pierre-Olivier; Rougeon, François and Lafaye, Pierre
FASEB Journal, 26(10) (pp. 3969-3979)
Transcriptional control of the multi-drug transporter ABCB1 by transcription factor Sp3 in different human tissues (2012-10)
Gromnicova, Radka; Romero, Ignacio A and Male, David
PLoS ONE, 7, Article e48189(10)
Transcellular targeting of fiber- and hexon-modified adenovirus vectors across the brain microvascular endothelial cells in vitro (2012-09)
Laakkonen, Johanna P.; Engler, Tatjana; Romero, Ignacio A; Weksler, Babette; Couraud, Pierre-Olivier; Kreppel, Florian and Kochanek, Stefan
PLoS ONE, 7(9) (e45977)
CCL2 disrupts the adherens junction: implications for neuroinflammation (2012-08)
Roberts, Toni K; Eugenin, Eliseo A; Lopez, Lillie; Romero, Ignacio A; Weksler, Babette B; Couraud, Pierre-Olivier and Berman, Joan W
Laboratory investigation; a journal of technical methods and pathology, 92(8) (pp. 1213-1233)
The involvement of a Na⁺- and Cl⁻-dependent transporter in the brain uptake of amantadine and rimantadine (2012-04-02)
Kooijmans, Sander A. A.; Senyschyn, Danielle; Mezhiselvam, Muguntha M.; Morizzi, Julia; Charman, Susan A.; Weksler, Babette; Romero, Ignacio-Andres; Couraud, Pierre-Olivier and Nicolazzo, Joseph A.
Molecular pharmaceutics, 9(4) (pp. 883-93)
Lamotrigine is a substrate for OCT1 in brain endothelial cells (2012-03-15)
Dickens, David; Owen, Andrew; Alfirevic, Ana; Giannoudis, Athina; Davies, Andrea; Weksler, Babette; Romero, Ignacio A; Couraud, Pierre-Olivier and Pirmohamed, Munir
Biochemical Pharmacology, 83(6) (pp. 805-814)
Hepatitis C Virus infects the endothelial cells of the blood-brain barrier (2012-03)
Fletcher, Nicola F.; Wilson, Garrick K.; Murray, Jacinta; Hu, Ke; Lewis, Andrew; Reynolds, Gary M.; Stamataki, Zania; Meredith, Luke W.; Rowe, Ian A.; Luo, Guangxiang; Lopez-Ramirez, Miguel A.; Baumert, Thomas F.; Weksler, Babette; Couraud, Pierre-Olivier; Kim, Kwang Sik; Romero, Ignacio A.; Jopling, Catherine; Morgello, Susan; Balfe, Peter and McKeating, Jane A.
Gastroenterology, 142(3) (pp. 634-643.e6)
CCL2 binding is CCR2 independent in primary adult human astrocytes (2012-02-09)
Fouillet, A.; Mawson, J.; Suliman, O.; Sharrack, B.; Romero, I. A. and Woodroofe, M. N.
Brain research, 1437 (pp. 115-26)
The transport of nifurtimox, an anti-trypanosomal drug, in an in vitro model of the human blood-brain barrier: Evidence for involvement of breast cancer resistance protein (2012-02)
Watson, Christopher P.; Dogruel, Murat; Mihoreanu, Larisa; Begley, David J.; Weksler, Babette B.; Couraud, Pierre O.; Romero, Ignacio A. and Thomas, Sarah A.
Brain research, 1436 (pp. 111-121)
Role of caspases in cytokine-induced barrier breakdown in human brain endothelial cells (2012)
Lopez-Ramirez, Alejandro; Fischer, Roman; Torres-Badillo, Claudia C.; Davies, Heather A.; Logan, Karen; Pfizenmaier, Klaus; Male, David K.; Sharrack, Basil and Romero, Ignacio A.
Journal of Immunology, 189(6) (pp. 3130-3139)
Uptake and cytotoxicity of citrate-coated gold nanospheres: Comparative studies on human endothelial and epithelial cells (2012)
Freese, Christian; Uboldi, Chiara; Gibson, Matthew I.; Unger, Ronald E.; Weksler, Babette B.; Romero, Ignacio A.; Couraud, Pierre-Olivier and Kirkpatrick, C. J.
Particle and Fibre Toxicology, 9, Article 23
Expression and localization of claudins-3 and -12 in transformed human brain endothelium (2012)
Schrade, Anja; Sade, Hadassah; Couraud, Pierre-Olivier; Romero, Ignacio A; Weksler, Babette B and Niewoehner, Jens
Fluids and barriers of the CNS, 9 (p 6)
Purine receptors and Ca2+ signalling in the human blood-brain barrier endothelial cell line hCMEC/D3 (2012)
Bintig , Willem; Begandt , Daniela; Schlingmann , Barbara; Gerhard , Linda; Pangalos , Maria; Dreyer , Lutz; Hohnjec , Natalija; Couraud , Pierre-Olivier; Romero, Ignacio A; Weksler, Babette B. and Ngezahayo , Anaclet
Purinergic Signalling, 8(1) (pp. 71-80)
Development of a three-dimensional, all-human in vitro model of the blood-brain barrier using mono-, co-, and tri-cultivation Transwell models (2011-08)
Hatherell, Kathryn; Couraud, Pierre-Olivier; Romero, Ignacio A.; Weksler, Babette and Pilkington, Geoffrey J.
Journal of neuroscience methods, 199(2) (pp. 223-229)
Tyrosine phosphorylation of VE-cadherin and claudin-5 is associated with TGF-β1-induced permeability of centrally derived vascular endothelium (2011-04)
Shen, Weiyong; Li, Shiying; Chung, Sook Hyun; Zhu, Ling; Stayt, Jason; Su, Tao; Couraud, Pierre-Olivier; Romero, Ignacio A.; Weksler, Babette and Gillies, Mark C.
European Journal of Cell Biology, 90(4) (pp. 323-332)
Uptake and permeability studies of BBB-targeting immunoliposomes using the hCMEC/D3 cell line (2011-02)
Markoutsa, Eleni; Pampalakis, Georgios; Niarakis, Anna; Romero, Ignacio A.; Weksler, Babette; Couraud, Pierre-Olivier and Antimisiaris, Sophia G.
European Journal of Pharmaceutics and Biopharmaceutics, 77(2) (pp. 265-274)
Surface characteristics of nanoparticles determine their intracellular fate in and processing by human blood–brain barrier endothelial cells in vitro (2011)
Georgieva, Julia V.; Kalicharan, Dharamdajal; Couraud, Pierre-Olivier; Romero, Ignacio A.; Weksler, Babette; Hoekstra, Dick and Zuhorn, Inge S
Molecular Therapy ((In Press))
Human brain endothelial cells are responsive to adenosine receptor activation (2011)
Mills, Jeffrey H.; Alabanza, Leah; Weksler, Babette B.; Couraud, Pierre-Olivier; Romero, Ignacio A. and Bynoe, Margaret S.
Purinergic Signalling, 7(2) (pp. 265-273)
Gliovascular and cytokine interactions modulate brain endothelial barrier in vitro (2011)
Chaitanya, Ganta V.; Cromer, Walter E.; Wells, Shannon R.; Jennings, Merilyn H.; Couraud, P. Oliver; Romero, Ignacio A; Weksler, Babette; Erdreich-Epstein, Anat; Mathis, James Michael; Minagar, Alireza and Alexander, J. Steven
Journal of Neuroinflammation, 8(162)
Insights into the putative catechin and epicatechin transport across blood-brain barrier (2011)
Faria, Ana; Pestana, Diogo; Teixeira, Diana; Couraud, Pierre-Olivier; Romero, Ignacio A; Weksler, Babette; de Freitas, Victor; Mateus, Nuno and Calhau, Conceição
Food & Function(2) (pp. 39-44)
A novel vascular targeting strategy for brain-derived endothelial cells using a TCR mimic antibody (2010-11)
Bhattacharya, Raktima; Xu, Yan; Rahman, Md. Ashequr; Couraud, Pierre-Olivier; Romero, Ignacio A.; Weksler, Babette B.; Weidanz, Jon A. and Bickel, Ulrich
Journal of Cellular Physiology, 225(3) (pp. 664-672)
ABC and SLC transporter expression and POT substrate characterization across the human CMEC/D3 blood-brain barrier cell line (2010-08-02)
Carl, Stephen M.; Lindley, David J.; Couraud, Pierre O.; Weksler, Babette B.; Romero, Ignacio; Mowery, Stephanie A. and Knipp, Gregory T.
Molecular Pharmaceutics, 7(4) (pp. 1057-1068)
Amyloid-β-induced occludin down-regulation and increased permeability in human brain endothelial cells is mediated by MAPK activation (2010-01)
Tai, L. M.; Holloway, K. A.; Male, D. K.; Loughlin, A. J. and Romero, I. A
Journal of Cellular and Molecular Medicine, 14(5) (pp. 1101-1112)
Differential activation of mitochondrial apoptotic pathways by vasculotropic amyloid-β variants in cells composing the cerebral vessel walls (2010)
Fossati, S.; Cam, J.; Meyerson, J.; Mezhericher, E.; Romero, I. A.; Couraud, P. O.; Weksler, B. B.; Ghiso, J. and Rostagno, A.
FASEB Journal, 24(1) (pp. 229-241)
Evaluation of soluble junctional adhesion molecule-a as a biomarker of human brain endothelial barrier breakdown (2010)
Najbauer, Joseph; Haarmann, Axel; Deiß, Annika; Prochaska, Jürgen; Foerch, Christian; Weksler, Babette; Romero, Ignacio; Couraud, Pierre-Olivier; Stoll, Guido; Rieckmann, Peter and Buttmann, Mathias
PLoS ONE, 5(10) (e13568)
Identification of peptide ligands for targeting to the blood-brain barrier (2010)
van Rooy, Inge; Cakir-Tascioglu, Serpil; Couraud, Pierre-Olivier; Romero, Ignacio A.; Weksler, Babette; Storm, Gert; Hennink, Wim E.; Schiffelers, Raymond M. and Mastrobattista, Enrico
Pharmaceutical Research, 27(4) (pp. 673-682)
Methamphetamine disrupts blood–brain barrier function by induction of oxidative stress in brain endothelial cells (2009-12)
Ramirez, Servio H.; Potula, Raghava; Fan, Shongshan; Eidem, Tess; Papugani, Anil; Reichenbach, Nancy; Dykstra, Holly; Weksler, Babette B.; Romero, Ignacio A.; Couraud, Pierre O. and Persidsky, Yuri
Journal of Cerebral Blood Flow and Metabolism, 29(12) (pp. 1933-1945)
Immortalized Human Brain Endothelial Cell Line HCMEC/D3 as a Model of the Blood-Brain Barrier Facilitates In Vitro Studies of Central Nervous System Infection by Cryptococcus neoformans (2009-11)
Vu, Kiem; Weksler, Babette; Romero, Ignacio A; Couraud, Pierre-Olivier and Gelli, Angie
Eukaryotic Cell, 8(11) (pp. 1803-1807)
Modulation of blood-brain barrier permeability by neutrophils: in vitro and in vivo studies (2009-10)
Joice, Shannon L.; Mydeen, Firdau; Couraud, Pierre-Olivier; Weksler, Babette B.; Romero, Ignacio A.; Fraser, Paul A. and Easton, Alexander S.
Brain Research, 1298 (pp. 13-23)
Polarized P-glycoprotein expression by the immortalised human brain endothelial cell line, hCMEC/D3, restricts apical-to-basolateral permeability to rhodamine 123 (2009-09-23)
Tai, Leon M.; Reddy, P. Sreekanth; Lopez-Ramirez, M. Alejandro; Davies, Heather A.; Male, A. David K.; Loughlin, A. Jane and Romero, Ignacio A.
Brain Research, 1292 (pp. 14-24)
Signaling mechanism of extracellular RNA in endothelial cells (2009-07)
Fischer, Silvia; Nishio, Miwako; Peters, Saskia C.; Tschernatsch, Marlene; Walberer, Maureen; Weidemann, Susanne; Heidenreich, Regina; Couraud, Pierre O.; Weksler, Babette B.; Romero, Ignacio A.; Gerriets, Tibo and Preissner, Klaus T.
FASEB Journal, 23(7) (pp. 2100-2109)
Meningococcal type IV pili recruit the polarity complex to cross the brain endothelium (2009-06-11)
Coureuil, Mathieu; Mikaty, Guillain; Miller, Florence; Lécuyer, Hervé; Bernard, Christine; Bourdoulous, Sandrine; Duménil, Guillaume; Mège, René-Marc; Weksler, Babette B.; Romero, Ignacio A.; Couraud, Pierre Olivier and Nassif, Xavier
Science, 325(5936) (pp. 83-87)
P-glycoprotein and breast cancer resistance protein restrict apical-to-basolateral permeability of human brain endothelium to amyloid-β (2009-06)
Tai, Leon M.; Loughlin, A. Jane; Male, David K. and Romero, Ignacio A.
Journal of Cerebral Blood Flow and Metabolism, 29(6) (pp. 1079-1083)
Expression of ADAM-17, TIMP-3 and fractalkine in the human adult brain endothelial cell line, hCMEC/D3, following pro-inflammatory cytokine treatment (2009-03-25)
Hurst, Louise A.; Bunning, Rowena A.D.; Couraud, Pierre-Olivier; Romero, Ignacio A.; Weksler, Babette B.; Sharrack, Basil and Woodroofe, M. Nicola
Journal of Neuroimmunology, 210(1-2) (pp. 108-112)
Expression of inflammatory genes induced by beta-amyloid peptides in human brain endothelial cells and in Alzheimer's brain is mediated by the JNK-AP1 signaling pathway (2009-03-18)
Vukic, Vanja; Callaghan, Debbie; Walker, David; Lue, Lih-Fen; Liu, Qing Yan; Couraud, Pierre-Oliver; Romero, Ignacio A.; Weksler, Babette; Stanimirovic, Danica B. and Zhang, Wandong
Neurobiology of Disease, 34(1) (pp. 95-106)
Up-regulation of P-glycoprotein by HIV protease inhibitors in a human brain microvessel endothelial cell line (2009-03-14)
Zastre, Jason A.; Chan, Gary N. Y.; Ronaldson, Patrick T.; Ramaswamy, Manisha; Couraud, Pierre O.; Romero, Ignacio A.; Weksler, Babette; Bendayan, Moise and Bendayan, Reina
Journal of Neuroscience Research, 87(4) (pp. 1023-1036)
Expression and transcriptional regulation of ABC transporters and cytochromes P450 in hCMEC/D3 human cerebral microvascular endothelial cells (2009-03)
Dauchy, Sandrine; Miller, Florence; Couraud, Pierre-Olivier; Weaver, Richard, J.; Weksler, Babette; Romero, Ignacio A.; Scherrmann, Jean-Michel; De Waziers, Isabelle and Decleves, Xavier
Biochemical Pharmacology, 77(5) (pp. 897-909)
Transcriptional control of occludin expression in vascular endothelia: regulation by Sp3 and YY1 (2009-03)
Sade, Hadassah; Holloway, Karen; Romero, Ignacio A. and Male, David
Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms, 1789(3) (pp. 175-184)
Expression of chemokines and their receptors by human brain endothelium: Implications for multiple sclerosis (2009-03)
Subileau, Eve A; Rezaie, Payam; Davies, Heather; Colyer, Frances; Greenwood, John; Male, David and Romero, Ignacio A
Journal of Neuropathology and Experimental Neurology, 68(3) (pp. 227-240)
Peripheral blood CD4+ T lymphocytes from multiple sclerosis patients are characterized by higher PSGL-1 expression and transmigration capacity across a human blood-brain barrier-derived endothelial cell line (2009)
Bahbouhi, Bouchaib; Berthelot, Laureline; Pettré, Ségolène; Michel, Laure; Wiertlewski, Sandrine; Weksler, Babette; Romero, Ignacio A.; Miller, Florence; Couraud, Pierre-Olivier; Brouard, Sophie; Laplaud, David-Axel and Soulillou, Jean-Paul
Journal of Leukocyte Biology, 86(5) (pp. 1049-1063)
Molecular cytogenetic characterization of the human cerebral microvessel endothelial cell line hCMEC/D3 (2009)
Mkrtchyan, H.; Scheler, S.; Klein, I.; Fahr, A.; Couraud, P. O.; Romero, I. A.; Weksler, B. and Liehr, T.
Cytogenetic and Genome Research, 126 (pp. 313-317)
The human brain endothelial cell line hCMEC/D3 as a human blood-brain barrier model for drug transport studies (2008-12)
Poller, Birk; Gutmann, Heike; Krahenbuhl, Stephan; Weksler, Babette; Romero, Ignacio A.; Couraud, Pierre-Olivier; Tuffin, Gerald; Drewe, Jürgen and Huwyler, Jörg
Journal of Neurochemistry, 107(5) (pp. 1358-1368)
Alteration of blood–brain barrier integrity by retroviral infection (2008-11-04)
Afonso, Philippe V.; Ozden, Simona; Cumont, Marie-Christine; Seilhean, Danielle; Cartier, Luis; Rezaie, Payam; Mason, Sarah; Lambert, Sophie; Huerre, Michel; Gessain, Antoine; Couraud, Pierre-Olivier; Pique, Claudine; Ceccaldi, Pierre-Emmanuel and Romero, Ignacio A.
PLoS Pathogens, 4, Article e1000205(11)
Chemokine production and chemokine receptor expression by human glioma cells: Role of CXCL10 in tumour cell proliferation (2008-08)
Maru, Seema V.; Holloway, Karen A.; Flynn, Geraldine; Lancashire, Christine L.; Loughlin, A. Jane; Male, David K. and Romero, Ignacio A.
Journal of Neuroimmunology, 199(1-2) (pp. 35-45)
Hyperosmotic stress induces axl activation and cleavage in cerebral endothelial cells (2008-07)
Wilhelm, Imola; Nagyoszi, Péter; Farkas, Attila E.; Couraud, Pierre-Olivier; Romero, Ignacio A.; Weksler, Babette; Fazakas, Csilla; Dung, Ngo Thi Khue; Bottka, Sándor; Bauer, Hannelore; Bauer, Hans-Christian and Krizbai, István A.
Journal of Neurochemistry, 107(1) (pp. 116-126)
Simvastatin Protects against Amyloid β and HIV-1 Tat-Induced Promoter Activities of Inflammatory Genes in Brain Endothelial Cells (2008-05)
András, I. E.; Rha, G.; Huang, W.; Eum, S.; Couraud, P. O.; Romero, I. A.; Henning, B. and Toborek, M.
Molecular Pharmacology, 73(5) (pp. 1424-1433)
Differential effects of hydrocortisone and TNFalpha on tight junction proteins in an in vitro model of the human blood-brain barrier (2008-04-01)
Förster, C.; Burek, M.; Romero, I. A.; Weksler, B.; Couraud, P. O. and Drenckhahn, D.
Journal of Physiology, 586(7) (pp. 1937-1949)
Immortalized human brain endothelial cells and flow-based vascular modeling: a marriage of convenience for rational neurovascular studies (2008-02)
Cucullo, Luca; Couraud, Pierre-Olivier; Weksler, Babette; Romero, Ignacio-Andres; Hossain, Mohammed M; Rapp, Edward and Janigro, Damir
Journal of Cerebral Blood Flow and Metabolism, 28(2) (pp. 312-328)
Activation of β-catenin signalling by GSK-3 inhibition increases p-glycoprotein expression in brain endothelial cells (2008)
Lim, Joseph C.; Kania, Katarzyna D.; Wijesuriya, Hasini; Chawla, Sangeeta; Sethi, Jaswinder K.; Pulaski, Lukasz; Romero, Ignacio A.; Couraud, Pierre O.; Weksler, Babette B.; Hladky, Stephen B. and Barrand, Margery A.
Journal of Neurochemistry, 106(4) (pp. 1855-1865)
Reactive oxygen species alter brain endothelial tight junction dynamics via RhoA, PI3 kinase, and PKB signaling (2007-11)
Schreibelt, G.; Kooij, G.; Reijerkerk, A.; van Doorn, R.; Gringhuis, S.I.; van der Poll, S.M.A.; Weksler, B.B.; Romero, Ignacio A; Couraud, P.O.; Piontek, J.; Blasig, I.E.; Dijkstra, Christine D.; Ronken, E. and de Vries, H.E.
FASEB Journal, 21(13) (pp. 3666-3676)
Regulation of cerebral endothelial cell morphology by extracellular calcium (2007-10-21)
Wilhelm, Imola; Farkas, Attila E.; Nagyoszi, Peter; Varo, Gyorgy; Balint, Zoltan; Vegh, Gergely A.; Couraud, Pierre-Olivier; Romero, Ignacio A.; Weksler, Babette and Krizbai, Istvan A.
Physics in medicine and biology, 52(20) (pp. 6261-6274)
Human Blood Brain Barrier Disruption by Retroviral Infected Lymphocytes: Role of Myosin Light Chain Kinase in Endothelial Tight-Junction Disorganization (2007-08-15)
Afonso, P.V.; Ozden, S.; Prevost, M.C.; Schmitt, C.; Seilhan, D.; Weksler, B.B.; Couraud, P.O.; Gessain, A.; Romero, I.A. and Ceccaldi, P.E.
Journal of Immunology, 179(4) (pp. 2576-2583)
Action of transcription factors in the control of transferrin receptor expression in human brain endothelium (2007-02-02)
Holloway, Karen; Sade, Hadassah; Romero, Ignacio A. and Male, David
Journal of Molecular Biology, 365(5) (pp. 1271-1284)
Blood-brain barrier-specific properties of a human adult brain endothelial cell line (2005-09)
Weksler, B.B.; Subileau, E.A.; Perriere, N.; Charneau, P.; Holloway, K.; Leveque, M.; Tricoire-Leignel, H.; Nicotra, A.; Bourdolos, S.; Tourowski, P.; Male, D.K.; Roux, F.; Greenwood, J.; Romero, I.A. and Couraud, P.O.
FASEB Journal, 19(13) (pp. 1872-1874)
Oxidative stress affects the junctional integrity of retinal pigment epithelial cells (2004-02)
Bailey, T. A.; Kanuga, N.; Romero, I. A.; Greenwood, J.; Luthert, P. J. and Cheetham, M. E.
Investigative Ophthalmology and Visual Science, 45(2) (pp. 675-684)
Secretion of interleukin-1β by astrocytes mediates endothelin-1 and tumour necrosis factor-α effects on human brain microvascular endothelial cell permeability (2003-07)
Didier, Nathalie; Romero, Ignacio A.; Creminon, Christophe; Wijkhuisen, Anne; Grassi, Jacques and Mabondzo, Aloise
Journal of Neurochemistry, 86(1) (pp. 246-254)
Changes in cytoskeletal and tight junctional proteins correlate with decreased permeability induced by dexamethasone in cultured rat brain endothelial cells (2003-06-26)
Romero, I.A.; Radewicz, K.; Jubin, E.; Michel, C.C.; Greenwood, J.; Couraud, P.O. and Adamson, P.
Neuroscience Letters, 344(2) (pp. 112-116)
Regulation of chemokine receptor expression in human microglia and astrocytes (2003-03)
Flynn, Geraldine; Maru, Seema; Loughlin, Jane; Romero, Ignacio A. and Male, David
Journal of Neuroimmunology, 136(1-2) (pp. 84-93)
Ezrin and moesin co-localise with ICAM-1 in brain endothelial cells but are not directly associated (2002-09-30)
Romero, I.A.; Amos, C.L.; Greenwood, J. and Adamson, P.
Molecular Brain Research, 105(1-2) (pp. 47-59)
Human adipose cells express CD4, CXCR4, and CCR5 receptors: a new target cell type for the immunodeficiency virus-1? (2002-08)
Romero, Ignacio A.; Hazan, Uriel; Cancello, Raffaella; Valente, Susana; Perrin, Virginie; Mariot, Virginie; Dumonceaux, Julie; Cindy C., Gerhardt; Strosberg, A. Donny; Courard, Pierre-Olivier and Pietri-Rouxel, France
FASEB Journal, 16(8) (pp. 1254-1256)
Chemokines control fat accumulation and leptin secretion by cultured human adipocytes (2001-04-25)
Gerhardt, C. C.; Romero, Ignacio A; Cancello, R.; Camoin, L. and Strosberg, A. D.
Molecular and cellular endocrinology, 175(1 - 2) (pp. 81-92)
Cross-linking of brain endothelial intercellular adhesion molecule (ICAM)-1 induces association of ICAM-1 with detergent-insoluble cytoskeletal fraction (2001)
Amos, Claire; Romero, Ignacio A.; Schulze, Charlotte; Rousell, Jonathan; Pearson, Jeremy D.; Greenwood, John and Adamson, Peter
Arteriosclerosis, Thrombosis, and Vascular Biology, 21 (pp. 810-816)
Age-related changes in blood-brain barrier integrity in C57BL/6J mice (2013)
Wang, C.; Saffrey, M. J. and Romero, I. A.
In : British Society for Research on Ageing Annual Scientific Meeting (2-4 September 2013, Institute of Food Research, University of East Anglia, Norwich) (p 36)
Effects of neurotrophic factors on neurochemical differentiation of postnatal rat myenteric plexus ganglion cells in vitro (2003)
Xeniou, Ourania; Romero, Ignacio A and Saffrey, Jill
In : British Neuroscience Association 17th National meeting (13-16 April, 2003, Harrogate, UK) (p 48)
The role of neurotrophic factors and extracellular matrix components in postnatal myenteric neuronal differentiation (2003)
Xeniou, O.; Romero, I. and Saffrey, M. J.
In : Enteric Nervous System 2003, An International Conference devoted to studies of the enteric nervous system (9-13 July, 2003, Banff, Alberta, Canada) (p 201)
Human Blood Brain Barrier Model (2006-06-01)
Couraud, P.O.; Romero, I.A. and Weksler, B.B.

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